Chemically Modified Glass & Mica Surfaces for Atomic Force Microscopy

Atomic Force Microscopes, AFM Force Spectroscopy / Measurement, Novascan UV Ozone Cleaners, AFM Bead probes, AFM Chemical Probes, AFM Spring Constant Calibration, Anti-Vibration Acoustic Isolation>
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Chemically Modified Surfaces for Atomic Force Microscopy

Novascan Technologies glass and mica surfaces with defined chemistries are designed for use in:

Atomic Force Microscopy (AFM)
Confocal Microscopy
TIRF (Total Internal Reflection Flourescence)
Protein Binding Studies
Chemical Binding Studies
NSOM/SNOM (Nearfield Scanning Optical Microscopy)

Modified surfaces are comprised primarily of either thiol-based or silane-based self assembled monolayers (SAMs). Thiol modified surfaces, available with CH₃, COOH, OH and succinimde end groups, are attached via chemical apsorption to a freshly applied layer of gold. Alternatively, silane monolayers, including APTES, are tethered to oxide layers on the surface of either glass or mica substrates. Further modifications can yield a variety of surface chemistries including NH₃, maleimide, biotin, streptavidin, neutravidin, antibodies, PEG linkers and a number of other molecules.

Standard mica surfaces are 1cm x 1cm although custom sizing is available. Glass surfaces are 12mm round pieces, however other sizes may also be available. Be sure to mention custom requirements when inquiring. As is always the case at Novascan, we welcome the opportunity to discuss custom modifications including custom particle attachment or chemical modifications.

Potential Applications

Inter-molecular Force Measurement
Chemical Sensing and Detection
Hydrophilic/Hydrophobic Interaction
Attractive/Repulsive Regimes
Adhesion Forces
Unbinding Forces
Surface Mapping

Diagram of AFM tip modified with silane

Diagram of AFM tip modified with Thiol

Surfaces with Defined Chemistry

Mica Surfaces

SUR.MICA.AP - Amino Silanized (APTES) mica
SUR.MICA.OH - Au coated mica with an OH surface
SUR.MICA.CH3 - Au coated mica with a CH₃ surface
SUR.MICA.COOH - Au coated mica with COOH Surface
SUR.MICA.SUC - Au coated mica with a succinimide surface
SUR.MICA.PEG.BIO - Mica surface with Biotin terminated flexible PEG linker
SUR.MICA.PEG.NH3 - Mica surface with NH₃ terminated flexible PEG linker
SUR.MICA.PEG.MAL - Mica surface with Maleimide terminated flexible PEG linker
SUR.MICA.PEG.COOH - Mica surface with COOH terminated flexible PEG linker

Glass Surfaces

SUR.GS.AP - Amino Silanized (APTES) Glass
SUR.GS.OH - Au coated glass with an OH Surface
SUR.GS.CH3 - Au coated glass with a CH₃ Surface
SUR.GS.COOH - Au coated glass with a COOH Surfaces
SUR.GS.SUC Succinimide Surfaces...
SUR.GS.PEG.BIO Surface with Biotin terminated flexible PEG linker
SUR.GS.PEG.NH3 Surface with NH₃ terminated flexible PEG linker
SUR.GS.PEG.MAL Surface with Maleimide terminated flexible PEG linker
SUR.GS.PEG.COOH Surface with COOH terminated flexible PEG linker